Figure 3.

Inhibition of autophagy abrogated the inhibitory effects of TRIM72 on NLRP3 activation. (a) Representative histology images and inflammatory response reflected by HE staining in tibial anterior muscles of AAV-TRIM72 mdx mice with or without CQ treatment. Scale bar, 100 μm. (b, c) Western blot and quantification of inflammasome-related proteins NLRP3, ASC, and IL1β expression in tibial anterior muscles of AAV-TRIM72 mdx mice with or without CQ treatment, n = 4. (d, e) Western blot and quantification of autophagy-related proteins P62 and LC3 expression in AAV-TRIM72 mdx mice with or without CQ treatment, n = 4. Data were expressed as mean ± SEM. ^∗p < 0.05, ^∗∗p < 0.01, and ^∗∗∗p < 0.001. CQ: chloroquine.