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. 2023 Jan 18;2023:8408574. doi: 10.1155/2023/8408574

Figure 4.

Figure 4

TRIM72 could scavenge mtROS through mitophagy induction in C2C12 cells. (a, b) Representative images and statistical data of MitoSOX Red fluorescence in C2C12 cells, LV-TRIM72 cells, and LV-TRIM72 + si-TRIM72 cells. Cells were cocultured with or without antimycin A (AA, 10 μM) and/or chloroquine (CQ, 50 μM) for 24 h. Scale bar, 50 μm. Data were obtained from 15 randomly selected cells from 3 independent experiments. (c) Representative immunofluorescence images of LC3, MitoTracker, and the merged images in C2C12 cells. Cells were cultured with or without CCCP (50 μM), LV-TRM72, or si-TRIM72 for 12 h. Scale bar, 20 μm. (d, e) Statistical data of colocalized LC3 and MitoTracker was analyzed by Pearson's coefficient or dots per cell. Data were obtained from 24 randomly selected cells from 3 independent experiments. Data were expressed as mean ± SEM. ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001. AA: antimycin A; CCCP: carbonyl cyanide m-chlorophenylhydrazone.