Fig. 1.

Molecular characterization and cytotoxic effects of AZD5363, AZD8542, CCR and RV in human GBM cells. (A) RT-PCR products for AKT (501 bp), SMO (452 pb) and β-actin (377 pb), SH-SY5Y neuroblastoma cells were used as positive controls. (B) Cytotoxic effect of AZD5363, AZD8542, CCR and RV on U-87 MG and A172 cell lines as assessed by the MTT assay. Bars are means ± SEM of three independent experiments. One-way ANOVA, followed by Tukey's, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. ns, non-significant. (C) IC50 values of AZD5363, AZD8542, CCR and RV for the U-87 MG and A172 cell lines.