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. 2023 Jan 20;33:101430. doi: 10.1016/j.bbrep.2023.101430

Fig. 5.

Fig. 5

Effect of the combined treatments with AZD5363+AZD8542+CCR or AZD8542+CCR+RV on the activity of the PI3K/AKT and SHH survival pathways on U-87 MG and A172 cell lines. (A) and (C) representative images of the Western blot analysis of pAKT, tAKT, p-p70 S6k, p-S6k, SMO, GLI1 on U-87 MG (upper panel) and A172 (lower panel), cell lines. (B) and (D) quantitative analysis of the western blots for pAKT, tAKT, p-p70 S6k, p-S6k, SMO, GLI1 on U-87 MG (upper panel) and A172 (lower panel) cells. Data obtained from three independent experiments. Actin was used as loading control. One-way ANOVA, followed by Tukey; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. ns, non-significant.