Fig. 6.

Effect of the combined treatments with AZD5363+AZD8542+CCR or AZD8542+CCR+RV on the cell-cycle and caspase-dependent apoptosis on U-87 MG and A172 cell lines. (A) and (C) are representative images of the Western blot analysis of p21, p27, cyclin-D1, procas-3 and cas-3 on U-87 MG (upper panel) and A172 (lower panel), cell lines. (B) and (D) quantitative analysis of the western blots for p21, p27, cyclin-D1, procas-3 and cas-3 on U-87 MG (upper panel) and A172 (lower panel) cells. Data obtained from three independent experiments. Actin was used as loading control. Statistical analysis was performed using One-way ANOVA, followed by Tukey. Statistical significance levels are indicated as: *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. ns, non-significant.