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. 2022 Nov 30;613(7945):721–728. doi: 10.1038/s41586-022-05569-1

Extended Data Fig. 4. DUF368 functional conservation and effects on PG composition and crosslinking.

Extended Data Fig. 4

(a and b): Alkaline growth of Δvca0040 V. cholerae bearing pBAD18 vectors expressing (a) SAOUHSC_00846 from S. aureus or (b) HVO_RS12060 from H. volcanii. (c-e): Composition and crosslinking analysis in the indicated V. cholerae strains at the indicated pH in M63 media. Suppressor: Δvca0040/ΔsecDF1. (f and g): Same as c-e for S. aureus grown in the indicated conditions. a, b: representative data from n = 3 cultures per strain/condition. c-g: mean ± SD from n = 3 cultures per strain/condition. Statistical analysis was performed with one-way ANOVAs with Tukey’s multiple comparison tests (c-e) or unpaired student’s two-tailed t-tests (f, g).