Extended Data Fig. 8. Reactivity of γδ T cells towards cancer cell lines in the presence of NKG2D ligand blocking.

a. Representative flow cytometry plots showing NKG2D expression on γδ T cells from a MMR-d colon cancer upon co-culture with cancer cell lines as compared to medium only. Gates indicate percentage of positive γδ T cells. b. Bar plots showing NKG2D expression on γδ T cells from MMR-d colon cancers (n = 5) upon co-culture with cancer cell lines. Medium as negative control and PMA/ionomycin as positive control are included. Bars indicate mean ± SEM. Data from at least two independent experiments except for CRC167 (SW403, SK-CO-1, K-562), depending on availability of γδ T cells. c. Bar plots showing the killing of HT-29 cells upon co-culture with γδ T cells with or without NKG2D ligand blocking. Bars indicate mean ± SEM of two wells with two images/well. d. Bar plots showing the expression of CD137 (first row), OX40 (second row), and PD-1 (third row) on γδ T cells upon co-culture of γδ T cells with CRC cell lines with or without NKG2D ligand blocking. PD-1 expression is shown as difference from baseline (medium) condition. Bars and lines indicate mean and similar experiments, respectively. Data from two independent experiments for CD137 and OX40.