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. 2023 Jan 11;613(7945):743–750. doi: 10.1038/s41586-022-05593-1

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 4 — a. FACS gating strategy for single, live CD3⁺ TCRγδ⁺ cells from a representative MMR-d colon cancer sample showing sequential gates with percentages. b. Sorting of all γδ T cells from CRC94 (due to the low number of PD-1⁺ cells), and of PD-1^– (blue squares) and PD-1⁺ (orange squares) γδ T cells from CRC167, CRC134, CRC96, and CRC154. Dots represent single cells. c. Table showing the number of γδ T cells isolated from MMR-d colon cancers (n = 5) at the time of sorting vs 3-4 weeks after expansion, and the fold increase thereof. d. TCR Vδ chain usage after expansion of γδ T cells from CRC94 and CRC167 (first row), and at the time of sorting (left panel) as well as after expansion (right panel) of γδ T cells from CRC134, CRC96, and CRC154. PD-1^– γδ T cells from CRC154 did not expand in culture. Dots represent single cells.

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