TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Description and relevant phenotype | Reference or source |
|---|---|---|
| Strains | ||
| DBS100 | C. rodentium ATCC 51459 | 48, 49 |
| DBS231 | DBS100 cured of 65-kb plasmid | 48, 49 |
| EPEC E2348/69 | Wild-type E. coli O127:H6 isolate | 17 |
| EHEC EDL933 | Wild-type E. coli O157:H7 isolate | 45 |
| Plasmids | ||
| pBluescript II SK(+) | Cloning vector, Ampr | Stratagene |
| pCR2.1-TOPO | PCR cloning vector, Ampr Kanr | Invitrogen |
| pCRII-TOPO | PCR cloning vector, Ampr Kanr | Invitrogen |
| pTOPO-eae/espB | DBS100 eae to espB cloned in pCR2.1-TOPO | This study |
| pCRII-ler/cscT | DBS100 ler to escT cloned in pCRII-TOPO | This study |
| pCRII-cscT/C | DBS100 escT to escC cloned in pCRII-TOPO | This study |
| pCRII-cscC/N | DBS100 escC to escN cloned in pCRII-TOPO | This study |
| pCRII-cscN/tir | DBS100 escN to tir cloned in pCRII-TOPO | This study |
| pCRII-cscC | C. rodentium escC clone in pCRII-TOPO | This study |
| pBS-CRP3EV | C. rodentium 3-kb plasmid cloned in pBluescript II SK(+) as EcoRV fragment | This study |
| pBS-CRP3HIII | C. rodentium 3-kb plasmid cloned in pBluescript II SK(+) as HindIII fragment | This study |