TABLE 2.
Nucleotide primers used in this study
| Primer | Nucleotide sequence (from 5′ to 3′) | Target regiona |
|---|---|---|
| EscC-F | GATATAGGACGAATTGTG | EPEC escC |
| EscC-R | ATTCGCTAGATGCAGATTTTATCGG | EPEC escC |
| EscN-F | GGGAATAATATCGAACTTAAAG | EPEC escN |
| EscN-R | AGGCAACCACTTTGAATAGGC | EPEC escN |
| EscR-F | GATATGTCTCAATTAATGACCATTGGC | EPEC escR |
| EscR-R | CATCAATTCACCACCAACAGAAATTCG | EPEC escR |
| EscT-F | ATGAATGAGATAATGACGGTCATAGTATC | EPEC escT |
| EscT-R | TCACTCATTAATCATGCTCGGTAACG | EPEC escT |
| EscV-F | GAGCGCGTTCGCAGGATG | EPEC escV |
| EscV-R | ATGCTCTGAAATCATTTACCG | EPEC escV |
| EspB-F | ATGAATACTATCGATAATACCAATG | EPEC espB |
| EspB-R | TTACCCAGCTAAGCGAGCCGCTTGC | EPEC espB |
| Ler-F | CATGCGGAGATTATTTATTATGAATATGG | EPEC ler |
| Ler-R | GTTAAATATTTTTCAGCGGTATTATTTC | EPEC ler |
| Tir-1 | ATGCCTATTGGTAACCTTGGT | EPEC tir |
| Binv-1 | CGACTGCTCGTGACCTTAATGACC | CR espB |
| Cler-2 | GCTGGGATATACTAATGTGCCTG | CR ler |
| Cr1-1 | GCTGCCTTTTATCTCTGATGCCG | CR rorf1 |
| CscC-1 | GTTCTCCAGTAAGCGTGATCC | CR escC |
| CscC-2 | CCGGTAAGACTATTCGAGGTG | CR escC |
| CscN-1 | CTGCAAGTTCTCGTGTAAGCAC | CR escN |
| CcsN-2 | CTGGCATGTATTGTGGGCAGTG | CR escN |
| CscT-1 | GCGATTCATCCTCTATTACTGGCG | CR escT |
| CscT-2 | CCCAGCGGTCTGAGTATGCAAAAG | CR escT |
| Deng18 | GAACTGGCGCTGTCAGTCGTGCTAC | CR espB |
| Finv-1 | GGCTGTAGCAGCGGCAAATCATAAGC | CR escF |
| Int-5 | GTCTAATCATATAAACCCGGC | CR eae |
| R2-inv1 | CCAATGAGCAGAAAGCTGCAGTTGTGG | CR rorf2/espG |
| Tir-2 | GCTTATACTACAACTTGGTTC | CR tir |
| Tir-10 | CAAGAAAGAACAAATAACAGGCC | CR tir upstream |
a
CR, C. rodentium LEE; EPEC, EPEC E2348/69 LEE.