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. 2023 Jan 25;14(1):59. doi: 10.1038/s41419-023-05598-2

Fig. 3. circSLC38A1 promotes migration and invasion capacities of BC cells in vitro.

Fig. 3

A Relative expression levels of circSLC38A1 and SLC38A1 mRNA in T24 cells treated with circSLC38A1 siRNA or corresponding negative control (si-NC). B Relative expression levels of circSLC38A1 and SLC38A1 mRNA in UM-UC-3 cells after transduction with circSLC38A1 overexpression plasmid or vector plasmid. C The cell viability of T24 cell lines transfected with si-circSLC38A1 or si-NC were recorded by RTCA system. D The cell viability of UM-UC-3 cell lines transfected with circSLC38A1 overexpression plasmid or vector plasmid were recorded by RTCA system. E, F Wound healing assay (E), transwell migration, and matrigel invasion assay (F) showing decreased cell invasion in circSLC38A1 knockdown T24 cells. Scale bar, 100 µm (E), 25 µm (F). G, H Wound healing assay (G), transwell migration and matrigel invasion assay (H) showing that increased cell invasion in circSLC38A1 overexpression UM-UC-3 cells. Scale bar, 100 µm (G), 25 µm (H). I The expression levels of E-cadherin, N-cadherin, Vimentin, and snail in BC cells with knockdown or overexpression of circSLC38A1 were detected by western blot. Data are presented as means ± standard deviation from three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001.