TABLE 2.
Primers used in this study
| Primera | Sequence | Amplification target |
|---|---|---|
| RT-PCR | ||
| Tfbp-29 | AAT CGC GGT AGG CAT TGT TG | 924-bp RT-PCR product from tbpA |
| Tfbp-38 | CGG GCG TGT TTT ACG ACG | |
| oVCU-61 | CCA AGT CTG CTG TTC CAA CCT C | 523-bp RT-PCR product from tbpB |
| oVCU-62 | AAA CCA TCC GCC CAA CTC TTC G | |
| oVCU-46 | GCC GGC GTA GAA ACT TCC CGC | 885-bp RT-PCR product from porB1a |
| oVCU-47 | GCG CAG ACC GAC ACC GCC GAG | |
| oVCU-63 | GAA GAG TTG GGC GGA TGG TTT GC | 336-bp RT-PCR product from the intergenic region between tbpB and tbpA |
| oVCU-64 | GTA TCC AAC TGT TTT TCC TGT GCT TGT CCG | |
| oVCU-110 | TAT CGG AAC GTA CCG GGT AGC | 416-bp RT-PCR product from gonococcal 16S rRNA (21) |
| oVCU-111 | GTA TTA CCG CGG CTG CTG GCA | |
| Cloning | ||
| oVCU-68 | GGG GGT CAC GAA AAG TGT AAA GCG A | 343-bp segment of tbpA (native tbpA) |
| oVCU-72 | TAA ACA GTC CGC CGT ATT TGT GGT T | |
| oVCU-84 | GGG GGT CAC GAA AAG TGT AAA GCG AAT CCG | In conjunction with oVCU-68, generated a 293-bp segment of tbpA (deleted tbpA) |
| AAA AAC GGG CCC CAA CCG CTT CCT C | ||
| oVCU-70 | CGA CGC TTA GGG GAA ACC GCT TCA G | 360-bp segment of tbpB (native tbpB) |
| oVCU-73 | GTC GGG TAG CGT TGC TGA AGT TGT C | |
| oVCU-85 | CGA CGC TTA GGG GAA ACC GCT TCA GCG GCA | In conjunction with oVCU-70, generated a 310-bp segment of tbpB (deleted tbpB) |
| AGG CGT TTC GAC TCG TCT TCT TTG A | ||
| cRT-PCR | ||
| oVCU-119 | TGA ATT GTA ATA CGA CTC ACT ATA GGG CGA | T7 promoter from pCRII |
| oVCU-75 | GAA CCC GCC TGT TTT TGA TTT GCA T | In conjunction with oVCU-68, generated a 293-bp RT-PCR product from native tbpA and a 243-bp RT-PCR product from deleted tbpA |
| oVCU-77 | GTG TCA ATT CAA CCG CAT CCA AAA C | In conjunction with oVCU-70, generated a 310-bp RT-PCR product from native tbpB and a 260-bp RT-PCR product from deleted tbpB |
| Real-time RT-PCR | ||
| tbpA-107F | CCG ATC CGC TTT CAT ACG A | 102-bp segment of tbpA |
| tbpA-209R | TGT TGC GTG CGT TCG AGT A | |
| tbpA-170T | AAC GGC ACT ACA TCG GCG GCA | Labeled, tbpA-specific probe |
| tbpB-162F | GAT GGA AAA GTT GCC GTT GTC | 87-bp segment of tbpB |
| tbpB-249R | CAC CTG GGC CGC TTG A | |
| tbpB-203T | CAG CAC CGC AAA TGG CAA TGC TC | Labeled, tbpB-specific probe |
a
Primers used in RT reactions are shown in bold type.