Fig. 1. A new combinatorial methodology of NAD⁺ measurement using laser-captured microdissection (LCM) and high-performance liquid chromatography (HPLC).

a A scheme showing the methodology. b–g Chromatograms measured at 259 nm of standard NAD⁺ (b) and NAD⁺ in LCM-collected DMH (c) are shown. Absorbance spectra (d, e) and purity measurements (f, g) of peaks of standard NAD⁺ (d, f) and NAD⁺ in LCM-collected DMH (e, g) are also presented. NAD⁺ peaks, identified by arrows (b, c), were eluted at 13.7 min.