Fig. 2. Local NAD⁺ levels in each hypothalamic nucleus do not change at 17 months of age, but decrease significantly in the ARC, VMH, and LH, but not in the DMH, by 22 months of age.

a NAD⁺ levels in the ARC, VMH, DMH, and LH of male mice at 3 and 17 months of age are shown (n = 7–9). b NAD⁺ levels in the ARC, VMH, DMH, and LH of male mice at 3 and 22 months of age are shown (n = 7–14) **p ≤ 0.01 and *p ≤ 0.05 by unpaired Student’s t-test. Error bars indicate s.e.m. ARC arcuate nucleus, VMH ventromedial hypothalamic nucleus, DMH dorsomedial hypothalamic nucleus, LH lateral hypothalamic nucleus.