Fig. 3. A single intraperitoneal injection of nicotinamide mononucleotide (NMN) significantly increases NAD⁺ levels in all hypothalamic nuclei.

NAD⁺ levels in the ARC, VMH, DMH, and LH are shown in male mice at 22 months of age at 30 min after NMN administration at the dose of 300 mg/kg NMN (n = 3). Control, age-matched mice were injected with PBS. *p ≤ 0.05 by unpaired Student’s t-test. Error bars indicate s.e.m.