Skip to main content
. 2001 Oct;69(10):6348–6363. doi: 10.1128/IAI.69.10.6348-6363.2001

FIG. 8.

FIG. 8

Expression and export of GS, SOD, and MDH by M. smegmatis glnA1 pNBV1-MsGS-MsSODA-MDH. (A) Growth of M. smegmatis glnA1 pNBV1-MsGS-MsSODA-MDH. (B, C, and D) Enzyme activity of GS, SOD, and MDH in the culture filtrate (●) and lysate (▪) over time. (E) SDS-PAGE analysis of culture filtrates (CF) and lysates (L). Tenfold more CF than L, based on the original culture volume, was loaded on the gels (at 39 and 47 h, 4 ml of CF and 0.4 ml of L; at 63 to 189 h, 2 ml of CF and 0.2 ml of L). The positions of the M. smegmatis GS, M. smegmatis SOD, and the M. tuberculosis MDH are indicated. M, molecular mass markers in kilodaltons.