TABLE 4.
Expression and export of GS from M. smegmatis
| M. smegmatis strain | Activity (mU
ml−1)a
|
Extracellular activity (%) | Relative activityb | |
|---|---|---|---|---|
| Culture filtrate | Cell lysate | |||
| pNBV1 | 6.3 | 87.9 | 6.7 | 1.00 |
| pNBV1 (10 mM L-Gln) | 0.4 | 17.9 | 2.3 | 0.19 |
| glnA1 pNBV1 (10 mM L-Gln) | 0.0 | 0.1 | 0.0 | 0.00 |
| glnA1 pNBV1-MsGS | 62.3 | 1,238.4 | 4.8 | 13.81 |
| glnA1 pNBV1-MtbGS | 13.1 | 520.6 | 2.5 | 5.67 |
| glnA1 pNBV1-StGS | 0.1 | 3.9 | 2.7 | 0.04 |
a
Activity is expressed in milliunits per milliliter of the original culture volume.
b
Based on the total GS activity in the culture filtrate and lysate. Values are relative to the activity of the wild-type strain (pNBV1).