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Total monocytes/macrophages from the lungs of CCL3−/− and CCL3+/+ mice (n = 10 to 12 per group per time point), following intratracheal challenge with K. pneumoniae. Lung monocytes/macrophages were isolated from enzymatically digested lungs as described in Materials and Methods. Monocyte/macrophage recovery from uninfected CCL3−/− mice (n = 5) was (19.9 ± 6.1) × 106 and was (18.6 ± 5.6) × 106 from CCL3+/+ mice (n = 7). Data are from three independent, matched experiments.
