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. Author manuscript; available in PMC: 2024 Feb 1.
Published in final edited form as: Transplantation. 2022 Nov 11;107(2):e47–e57. doi: 10.1097/TP.0000000000004398

Figure 2. B cells are dispensable for graft CD4 T infiltration after allogeneic kidney transplantation.

Figure 2.

(A) Serum creatinine levels in C57BL/6 WT and uMT recipients of Balb/c kidney transplants at day 20 post-transplantation. (B) DSA measurements for IgG and IgM isotypes expressed as mean fluorescence intensity (MFI). Dotted line indicates the level of detection using a C57BL/6 probe for background. (C, D) Quantification of CD19+ B cells and CD4+ T percentage in draining lymph nodes and count in the grafts by flow cytometry. Representative gating (upper) and quantification (low) are shown. (E) Histological images of transplanted kidneys from HE staining, immunohistochemistry (IHC) staining for CD3 expression and immunofluorescent (IF) staining for C4d expression. Below: is histopathological scoring of intimal arteritis based on the Banff 2017 and the C4d positive signal score (0, −; 1, +; 2, ++; 3, +++). Magnification: 100×, scale bars: 100 μm. Data are from combined experiments with n=4–5 mice per group. Statistics: Student’s 2-tailed unpaired T test, **: P < 0.01; ***: P < 0.001.