Table 2.
Virus Summary Statistics. PMMoV, raw SARS-CoV-2 RNA, and PMMoV-normalized SARS-CoV-2 are compared via mean genome copy and standard deviation of each community. Quantification determined by plotting the inverse of the starting quantity of each virus’ standard curve on measured Cq via RT-qPCR. PMMoV is a stable, low-variability virus that is commonly excreted by humans. Normalization with PMMoV accounts for possible dilution or excess population waste contribution at each site on a per sample basis. N1 quantification of Community C was larger than Community A (Wilcoxon, p < 0.05) after population adjustment using PMMoV using the two normalization strategies.
| Community A | Community B | Community C | |
|---|---|---|---|
| Mean PMMoV genome copies per PCR reaction (Standard Deviation) | 73,650 (41,352) | 57,303 (45,010) | 38,622 (21,566) |
| N1 SARS-CoV-2 | |||
| Mean Raw SARS-CoV-2 genome copies per L (Standard Deviation) | 8150 (4010) | 18,150 (23,779) | 33,057 (32,138) |
| Mean PMMoV-Normalized SARS-CoV-2 per site PMMoV median (Standard Deviation) | 7905 (3462) | 17,674 (13,790) | 36,562 (34,992) |
| Mean PMMoV-Normalized SARS-CoV-2 per PMMoV RNA (Standard Deviation) | 0.1107 (0.0485) | 0.3619 (0.2824) | 1.016 (0.9723) |