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. Author manuscript; available in PMC: 2024 Jan 23.
Published in final edited form as: Curr Biol. 2022 Dec 16;33(2):215–227.e3. doi: 10.1016/j.cub.2022.11.039

Figure 6: Learning shapes the output of MBONs during eating.

Figure 6:

A) Left, Graphic and connectome reconstruction of β’mp axons of MB011B MBONs; in the cartoon, only cells in one hemisphere are shown; dotted line shows the region imaged. Right, Schematic of the appetitive conditioning protocol under the 2-photon microscope in pre-training (naive, green), training (yellow), and post-training (testing, blue) phases, 30 minutes apart, one before (light pink) and one after eating 2M sucrose (dark pink); yellow bulbs represent imaging and blue puffs air.

B-C) The calcium responses to MCH and OCT (puff) in the β′2mp axons of MB011B>GcAMP6f neurons post training but before (light pink, fasted) or after (dark pink, fed) consuming sucrose in Control Diet, CD (B) and Sugar Diet, SD (C) flies; n=16, data are shown as mean +/− SEM, B) ****p<0.0001 CD fasted CS+ vs fasted CS− (student’s t-test) and CD fasted CS+ vs fed CS+ (Wilcoxon test); ns, CD fed CS+ vs fed CS− (Mann-Whitney test), CD fed CS+ vs fed CS− (Wilcoxon test); C) respective comparisons ns, (Student’s t-test).

See also Figure S6.