Fig. 4 |. Mann-NC induces T_H17-associated cytokines and chemokines and promotes CD8⁺ T-cell and NK cell responses.

a, BALB/c mice were inoculated subcutaneously (s.c.) with 1.5 × 10⁵ CT26 cells on day 0 and treated by intra-tumoural (i.t.) administration of Mann-NC on days 9 and 12. Tumour tissues were analysed after 3 days of each treatment. b, Intra-tumoural concentrations of IL-6, IL-17, IL-23 and IL-1β. c,d, Intra-tumoural (c) and serum (d) concentrations of CXCL10, CCL2, CCL3 and CCL5 measured on day 15 (c) and on day 9 (d). e, Intra-tumoural frequencies of CD8⁺ T cells, AH-1 tetramer⁺ CD8⁺ T cells, the ratio of CD8⁺ T cells to T_reg cells, and the expression levels of IFN-γ, TNF-α and granzyme B. f, IFN-γ⁺ ELISPOT analysis performed on day 15 after restimulation of splenocytes with AH-1 peptide. g, Intra-tumoural frequencies of NK cells and their expression levels of CD69 and CD107a. Data represent mean ± s.e.m., from a representative experiment (n = 7 (b,c,e–g) and n = 12 (d) biologically independent samples) from two (b–d,f) or three (e,g) independent experiments. ***P < 0.001, ****P < 0.0001, analysed by two-way ANOVA with Bonferroni’s multiple comparisons test (b,d,e,g) or unpaired two-tailed Student’s t-test (c,f).