Fig. 5 |. Mann-NC induces T_H17-associated cytokines and chemokines and promotes CD8⁺ T-cell and NK cell responses.

a, BALB/c mice were inoculated subcutaneously (s.c.) with 1.5 × 10⁵ CT26 cells on day 0 and treated by intra-tumoural (i.t.) administration of Mann-NC. b,c, Isotype IgG or antibodies against αCD8, α-asialo-GM1, αLy6G (b) or α-IL-17A (c) were administered intraperitoneally (i.p.) on the indicated days, followed by tumour monitoring. d, IFN-γ⁺ ELISPOT analysis of splenocytes after restimulation with AH-1 or ME1 peptide. e,f, Shown are the frequency of total CD8⁺ T cells, IFN-γ⁺ CD8⁺ T cells and granzyme B⁺ CD8⁺ T cells (e), total NK cells, IFN-γ⁺ NK cells, and granzyme B⁺ NK cells in the TME (f). Data represent mean ± s.e.m., from a representative experiment (n = 5 for PBS or 7 for all other groups (b); n = 8 (c); or n = 7 (d–f) biologically independent samples) from one (d) or three (b,c,e,f) independent experiments. ***P < 0.001, ****P < 0.0001, analysed by one-way (d) or two-way (b,c) ANOVA with Bonferroni’s multiple comparisons test or unpaired two-tailed Student’s t-test (e,f).