FIGURE 1.

Cryo‐EM data processing of GPR21. (A) Representative micrograph of the complex particles. (B) Representative 2D images. (C) Workflow for cryo‐EM image processing. Gold standard Fourier shell correlation (FSC) curve indicates overall nominal resolution at 2.91 Å using the FSC = 0.143 criterion. (D) A cartoon representation of cryo‐EM 3D reconstruction of full‐length GPR21 (2.91 Å). GPR21 in complex with the human DNGαs, Gβ1 and Gγ2 together with G protein complex‐stabilizing nanobody Nb35. (E) Representative density maps and models for TM1‐7, H8 and ECL2 of GPR21