TABLE 1.
Cryo‐EM data collection, refinement, and validation statistics
| Data collection and processing | GPR21‐Gs‐Nb35 |
|---|---|
| Magnification | 105,000 |
| Voltage (kV) | 300 |
| Electron exposure (e–/Å) | 45.51 |
| Defocus range (μm) | −1.0 to −2.0 |
| Pixel size (Å) | 0.85 |
| Symmetry imposed | C1 |
| Initial particle images (no.) | 393,876 |
| Final particle images (no.) | 149,012 |
| Map resolution (Å) | 2.91 |
| FSC threshold | 0.143 |
| Refinement | |
| Model resolution (Å) | 2.91 |
| Map sharpening B factor (Å) | −89.6 |
| Model composition | |
| Nonhydrogen atoms | 8067 |
| Protein residues | 1015 |
| B factors (Å) | |
| Protein | 57.74 |
| r.m.s. deviations | |
| Bond lengths (Å) | 0.002 |
| Bond angles (°) | 0.439 |
| Validation | |
| MolProbity score | 1.2 |
| Clashscore | 4.12 |
| Ramachandran plot | |
| Favored (%) | 98.29 |
| Allowed (%) | 1.71 |
| Disallowed (%) | 0 |