TABLE 2.

De novo mutations identified by WGS in predicted off-target regions.

	Chr	Position	Gene	Sequence (5′-3′)	Blastomeres with de novo variants
gRNA 1	2	101,400,689–101,400,711	CCR5*	GCTGCT​GCC​CAG​TGG​GAC​TT
	1	63,497,146–63,497,168	NFASC	$\text{T}$ C $\text{A}$ ​GCT​GCC​CAG​TG $\text{T}$ ​GAC​TT	4-4, 4-5, 5-3, 5-4, 5-7, 5-8, 5-9
	1-scaffold	378,063–378,085	intergenic_ke145507.1	GCT​GCT​GCC​CAG​T $\text{A}$ G​ $\text{TG}$ C​TT	4-4, 4-6
	10	31,267,650–31,267,672	intergenic_CM001928.1	$\text{T}$ CT​GCT​GCC​CAG​TGG​G $\text{C}$ C​T $\text{C}$	5-1
	19	40,019,282–40,019,304	DLL3	GC $\text{CC}$ CT​GC $\text{A}$ ​CAG​TGG​GAC​TT	4-4, 4-6, 5-3
gRNA 2	2	101,400,887–101,400,909	CCR5*	GCT​GTG​TTT​GCC​TCT​CTC​CC
	7	35,664,631–35,664,653	LIPC	GCT​GTG​ $\text{C}$ TT​GCC​TCT​ $\text{T}$ TC​CC	4-5, 4-6, 5-1
	9	7,497,178–7,497,200	SFMBT2	GCT​G $\text{GC}$ ​TTT​GCC​TCT​CTC​CC	4-3, 4-6, 5-5, 5-7, 5-8
	11	129,547,583–129,547,605	intergenic_CM001929.1	GCT​GTG​ $\text{C}$ TT​ $\text{C}$ CC​TCT​CTC​CC	4-5, 4-6, 5-1, 5-5
	14	14,725,950–14,725,972	intergenic_CM001932.1	GCT​GTG​TT $\text{CT}$ CC​TCT​CTC​CC	4-5, 4-6, 5-4
	1	199,634,457–199,634,479	intergenic_CM001919.1	GCT​GTG​ $\text{G}$ TT​ $\text{T}$ CC​TCT​CTC​C $\text{T}$	5-3
	3	53,360,905–53,360,927	intergenic_CM001921.1	$\text{C}$ CT​GTG​ $\text{A}$ TT​ $\text{A}$ CC​TCT​CTC​CC	4-3, 4-5, 5-1, 5-5
	4	2,457,033–2,457,055	intergenic_CM001922.1	GCT​GTG​T $\text{C}$ T​ $\text{C}$ CC​TCT​C $\text{C}$ C​CC	5-1
	5	188,649,889–188,649,911	intergenic_CM001923.1	$\text{AA}$ T​GTG​TTT​GCC​TCT​ $\text{T}$ TC​CC	4-3, 5-1, 5-4, 5-7, 5-8, 5-9
	7	28,263,588–28,263,610	CYP19A1	G $\text{GA}$ ​GTG​TT $\text{G}$ ​GCC​TCT​CTC​CC	5-7
	12	62,901,723–62,901,745	intergenic_CM001930.1	GCT​GTG​T $\text{CC}$ ​GCC​TCT​CTC​ $\text{G}$ C	4-1, 4-3, 5-3, 5-5, 5-8
	17	62,039,482–62,039,504	NDFIP2	GCT​GT $\text{C}$ ​TTT​GCC​ $\text{A}$ CT​CTC​ $\text{A}$ C	4-3
	19	49,110,480–49,110,502	CCDC114	GCT​GTG​ $\text{CA}$ T​ $\text{C}$ CC​TCT​CTC​CC	5-1, 5-3, 5-4, 5-8

A total of 93 regions were assessed that contained 2-3 mismatches. *Denotes on-target gene sequence. Chr: chromosome.