FIG. 5.

iNOS distribution following phagocytosis in mouse primary peritoneal macrophages. Cells treated with IFN-γ and LPS were used in all panels. (A and B) Uptake of latex beads at 30 min after addition. iNOS staining (mouse monoclonal) in green is shown alone (A) and also merged with the differential interference contrast (DIC) image of the same cell (B). (C) A cell, 2 min after addition of zymosan particles, stained for iNOS (mouse monoclonal) in green. Accumulation of iNOS at the tips of pseudopodia closing around zymosan just entering the cell is shown by arrows. (D to F) Cells, 2 h after being fed latex beads for 10 min, which were then removed by washing. The panels show the pattern of iNOS staining (mouse monoclonal) in green (D), LAMP-1 staining in red (E), and the merged images of panels D and E (F), where areas of colocalization are shown in yellow. Two areas showing apparent accumulation of iNOS around the outside of phagocytosed beads are indicated by arrows in panel D. (G and H) Uptake of IgG-coated magnetic beads at 5 min after addition. iNOS staining (rabbit polyclonal antibody) in green is shown alone (G) and merged with the DIC image of the same cell (H). (I) A cell, 10 min after the addition of complement-coated zymosan particles stained for iNOS (mouse monoclonal) in green. Magnification, ×2,000 for all panels.