TABLE 1.

Overview of the described methods to measure blood flow and vascular reactivity in the retina.

	Main content	Principle of measurement	In vivo/Ex vivo	Commercially available
RVA	Vessel diameter	Fundus camera-based imaging technology	In vivo	Yes
LDV	Blood flow velocity	Optical Doppler shift	In vivo	Yes
LDF	Retinal blood flow	Scattering theory for light	In vivo	Yes
Dye-based Angiography	Visualization of anatomic structures	Passage of a fluorescent dye	In vivo	Yes
OCTA	Depth resolved angiograms	Detection of intravascular moving particles as an intrinsic contrast	In vivo	Yes
Doppler OCTA	Retinal blood flow	Generation of a reflective profile and detection of phase shifts of the back-scattered lights	In vivo	Yes
Retinal oximetry	Oxygen saturation of hemoglobin in RBCs	Difference in absorption of light between oxyhemoglobin and deoxyhemoglobin	In vivo	Yes
LSFG	Mean Blur Rate (MBR) as relative index of retinal blood flow velocity	Detection of changes in the speckle pattern by reflection of coherent laser light	In vivo	Yes
RBC labeling in vivo	Vessel diameter and passage of fRBCs per second	Detection of fluorescently labeled RBCs by ultrafast confocal line scans	In vivo	No
Transmitted light Microscopy ex vivo	Vessel diameter	Retinal preparation and measurement of retinal blood vessel reactivity by transmitted light microscopy	Ex vivo	No