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. 2001 Oct;69(10):6419–6426. doi: 10.1128/IAI.69.10.6419-6426.2001

FIG. 1.

FIG. 1

Purified S-pilin migrates as a tetrameric unit under nondenaturing conditions. (A) Immunoblot of gonococcal whole-cell lysates using PilE-specific antiserum. Lanes: 1, MS11-8, a PilC−, S-pilin producing derivative of MS11; 2, MS11-P−n. (B) Concentrated culture supernatants of MS11-P−n (lane 1) and MS11-8 (lane 2). Lane 3, purified S-pilin preparation of MS11-8. The proteins were separated by SDS–15% PAGE and stained with Coomassie brilliant blue. (C) S-pilin separated on a nondenaturing polyacrylamide gel and stained with Coomassie brilliant blue. S-pilin migrates as a tetrameric unit. Shown on the left are molecular size markers (kilodaltons). N-terminal sequencing of the 16-kDa protein showed that the 16-kDa product is S-pilin.