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Inhibition of S-pilin binding to human endometrial tissue. (A) Tissue stained with hematoxylin-eosin. (B) Tissue overlaid with purified S-pilin preparation of MS11-8 (1.5 μg/ml). (C) Preincubation of the tissue with proteinase K prior to addition of the MS11-8 S-pilin preparation. Bound S-pilin was detected with PilE antiserum and FITC-conjugated anti-rabbit IgG. The low fluorescence level still detected represents the background of the FITC-labeled secondary antibody.
