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Purified gonococcal S-pilin binds to a protein of the same size as CD46. SDS-PAGE of whole-cell lysates blotted onto a nitrocellulose membrane. Lanes: 1, ME180 cells overlaid with DIG-labeled S-pilin MS11-8; 2, ME180 cells overlaid with CD46 antiserum and AP-conjugated IgG antibodies; 3, CHO cells overlaid with DIG-labeled S-pilin of MS11-8.
