TABLE 2.
Chemical and enzymatic pretreatments of human endometrial tissue sections
| Pretreatment | Fluorescence level after addition of:
|
|||
|---|---|---|---|---|
| S-pilins 8, 6, 5, 3a | P− n supernatantb | Control lectinc | Control MCP antiserumd | |
| Proteinase K | − | − | +++ | − |
| PBS (control) | +++ | − | +++ | +++ |
| Periodate | +++ | − | − | +++ |
| Na acetate (control) | +++ | − | − | +++ |
| Neuraminidase | +++ | − | − | +++ |
| PBS (control) | +++ | − | +++ | +++ |
| Neuraminidase + periodate | +++ | − | − | +++ |
| PBS-Na acetate (control) | +++ | − | +++ | +++ |
a
Purified S-pilin at 2 μg/ml was added. +++; high fluorescence; −, low fluorescence.
b
Concentrated P− n supernatant at 20 μg/ml was added. −, low fluorescence.
c
The control for periodate treatment was the Dolichos biforus agglutinin lectin recognizing N-acetyl-α-d-galactosaminyl (α-d-GalNAc). The control for neuraminidase treatment was the lectin from Maakia amurensis recognizing glycoconjugate side chains containing sialic acid α2-3Gal. +++, strong lectin binding; −, no lectin binding detected.
d
MCP (CD46) antiserum, followed by FITC-conjugated antibody, was used to detect protein. +++, strong fluorescence; −, weak fluorescence.