Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Nov 10;95(1):e28266. doi: 10.1002/jmv.28266

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2022 Wiley Periodicals LLC.

This article is being made freely available through PubMed Central as part of the COVID-19 public health emergency response. It can be used for unrestricted research re-use and analysis in any form or by any means with acknowledgement of the original source, for the duration of the public health emergency.

PMC Copyright notice

Irradiation enhances sphingolipid metabolism by upregulating acid sphingomyelinase (ASM). (A) Volcano figure of the RNA‐seq data. Shown are the altered genes in irradiated A549 cells after 24 h. (B) Top five enriched pathways in Kyoto Encyclopedia of Genes and Genomes analysis. (C) Messenger RNA expressions of ASM in irradiated A549 cells at different times. Results are shown as the mean ± SD. ***p < 0.001, relative to the sham group. (D) Western blotting analysis revealing the ASM expressions in irradiated A549 cells. β‐actin was used as the reference. Histogram shows the results of gray analysis for the protein bands. Results are shown as the mean ± SD. ***p < 0.001, relative to the sham group. (E) Immunofluorescence microscopy revealing the expressions of ASM (red) in A549 cells. Nuclei were stained with DAPI (blue). The scale bar indicates 20 μm. SD, standard deviation.