Table 7.
Antibody level thresholds as measured by the Roche and DiaSorin immunoassays predicting detectability of spike‐binding neutralizing antibodies in vaccinated, SARS‐CoV‐2 naïve participants according to SARS‐CoV‐2 (sub)variant
| Immunoassay | SARS‐CoV‐2 (sub)varianta | Antibody threshold (BAU/ml) | Sensitivity (%) | Specificity (%) | AUC (p value) |
|---|---|---|---|---|---|
| Roche | Wuhan‐Hu‐1 | >300 | 90.54 | 92.86 | 0.96 (<0.001) |
| Omicron BA.1 | >4123 | 92.31 | 91.84 | 0.95 (<0.001) | |
| Omicron BA.2 | >8851 | 69.05 | 87.50 | 0.77 (0.012) | |
| DiaSorin | Wuhan‐Hu‐1 | >586 | 77.03 | 92.86 | 0.95 (<0.001) |
| Omicron BA.1 | >1505 | 79.49 | 91.84 | 0.94 (<0.001) | |
| Omicron BA.2 | >3055 | 61.90 | 87.50 | 0.72 (0.05) |
Abbreviations: AUC, area under the receiver operating characteristic curve; BAU, binding antibody units.
a
A total of 88 were assayed for quantitation of neutralizing antibodies against Wuhan‐Hu‐1 and Omicron BA.1. A total of 50 sera were assayed for neutralizing antibodies against Omicron BA.2.