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. 2023 Jan 1:10.1111/acer.15000. Online ahead of print. doi: 10.1111/acer.15000

FIGURE 2.

FIGURE 2

Effect of chronic intermittent EtOH (CIE) exposure on the ACE2, TMPRSS2, ADAM17, and Cathepsin L expression in hypothalamic nuclei. Representative confocal images of ACE2 positive cells in (A, D) PVN, (B, E) LH and (C, F) ARC of air control and CIE mice. Scale bar = 100 μm. (G–I) Histograms showing the % ACE2 immunoreactive area in PVN, LH, and ARC. (J) Representative Western blot (WB) and histogram showing the cACE2 and sACE2 protein levels in the hypothalamus. (K) Representative WB and histogram showing the TMPRSS2, ADAM17, and Cathepsin L protein levels in the hypothalamus. (L) Histogram showing the ACE2, ADAM17, and Cathepsin L mRNA levels in the hypothalamus. Values (n = 4 to 5/group for IF and n = 7‐8/group for WB and RT‐qPCR) are represented as mean (±SEM) and the data were analyzed by unpaired Student's t‐test with Welch's correction (*p < 0.05, **p < 0.01, ***p < 0.001 vs. air control).