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. 2023 Jan 1:10.1111/acer.15000. Online ahead of print. doi: 10.1111/acer.15000

FIGURE 3.

FIGURE 3

Effect of chronic intermittent EtOH (CIE) exposure on the 5HT, ACE2, and TMPRSS2 immunoreactivity in dorsal raphe nucleus (DRN). Representative confocal images showing (A, B) 5‐HT in the DRN (blue; scale bar = 100 μm) and (C, D) a single tile image (scale bar = 50 μm) from the boxed area of air control and CIE mice. (E, F) Representative images of ACE2 (red; scale bar = 100 μm) in the DRN and (G, H) a single tile image (scale bar = 50 μm) from the boxed area in air control and CIE mice. (I, J) TMPRSS2 (green; scale bar = 100 μm) in the DRN and (K, L) a single tile image (scale bar = 50 μm) from the boxed area in air control and CIE mice. (M, N) 5HT‐ACE2 (magenta) colocalization in the DRN and (O, P) a single tile image from the boxed area in air control and CIE mice. (Q, R) 5HT‐TMPRSS2 (white) positive neurons in the DRN and (S, T) a single tile image from the boxed area of air control and CIE mice. Histogram showing (U) % immunoreactive area for 5HT, ACE2, and TMPRSS2, (V) % immunoreactive area for 5HT‐ACE2 positive neurons, (W) Pearson correlation coefficient for 5HT‐ACE2 colocalization (X) Mander's overlap coefficient for 5HT‐ACE2 colocalization in DRN. Values (n = 4 to 5/group) are represented as mean (±SEM) and the data were analyzed by unpaired Student's t‐test with Welch's correction (*p < 0.05 vs. air control).