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. 2023 Jan 1:10.1111/acer.15000. Online ahead of print. doi: 10.1111/acer.15000

FIGURE 6.

FIGURE 6

Effect of chronic intermittent EtOH (CIE) exposure on the ACE2, TH, ADAM17, TMPRSS2, and Cathepsin L expression in locus coeruleus (LC). Representative confocal images showing (A, D) TH (green) (B, E) ACE2 (red) (C, F) TH‐ACE2 (yellow) positive neurons in LC of air and CIE mice. Scale bar = 50 μm. Graph showing (G) % immunoreactive area for ACE2 and TH, (H) % immunoreactive area for TH‐ACE2 positive neurons, (I) Pearson correlation coefficient for TH‐ACE2 colocalization (J) Mander's overlap coefficient for TH‐ACE2 colocalization in LC. (K) Representative Western blot (WB) and histogram showing the cACE2 and sACE2 protein levels in the LC. (L) Representative WB and histogram showing the TMPRSS2, and Cathepsin L protein levels in the LC. (M) Histogram showing the ACE2, ADAM17, and Cathepsin L mRNA levels in the LC. Values (n = 4 to 5/group for IF and n = 7‐8/group for WB and RT‐qPCR) are represented as mean (±SEM) and the data were analyzed by unpaired Student's t‐test with Welch's correction (*p < 0.05, **p < 0.01 vs. air control).