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. 2023 Jan 1:10.1111/acer.15000. Online ahead of print. doi: 10.1111/acer.15000

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© 2022 Research Society on Alcoholism.

This article is being made freely available through PubMed Central as part of the COVID-19 public health emergency response. It can be used for unrestricted research re-use and analysis in any form or by any means with acknowledgement of the original source, for the duration of the public health emergency.

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ACE2 distribution in neurons, microglia, and astrocyte in medullary raphe nuclei. The raphe magnus (RMG), and raphe obscurus (ROB) were stained for neuronal marker NeuN (panel A, F); microglia marker Iba1 (panel B, G); astrocyte marker GFAP (panel C, H) and ACE2 (panel D, I). Panel E shows the merged image of the RMG stained with all the markers (panels A–C) with ACE2 (panel D). Panel J shows the merged image of the ROB stained with all the markers (panels F–H) with ACE2 (panel I). Yellow arrows indicate the colocalization of ACE2 and Iba1 (yellow in the merge panels) and white arrows indicate the colocalization of ACE2 and NeuN (magenta in the merge panels). Scale bar = 100um.