TABLE 1.
List of primers used for quantitating mRNA expression using RT‐qPCR
| Gene name | Forward/reverse (5′‐3′) | Sequence |
|---|---|---|
| β‐Actin | Forward | CCAGCCTTCCTTCTTGGGTA |
| Reverse | GAGGTCTTTACGGATGTCAACG | |
| Ace2 | Forward | TGGGACACGGAGACTTCAGA |
| Reverse | TGGCTCCGTTTCTTAGCAGG | |
| Tmprss2 | Forward | TGAAACGCCAGAGCAGGATT |
| Reverse | AAATGCCGTCCAGTACCTCG | |
| Adam17 | Forward | GAAGACCCCAGCACAGACTC |
| Reverse | CCTTTTCGCTCCTGGTGACT | |
| Cathepsin L | Forward | GTCTTGTTGGGCGTTTAGCG |
| Reverse | TGAGCGTGAGAACAGTCCAC | |
| At1r | Forward | ACAACTGCCTGAACCCTCTG |
| Reverse | ACCTCAGAACAAGACGCAGG | |
| At2r | Forward | TTTGTTCTGGGCTTCGTCCC |
| Reverse | CTTGCCATTTGCTGGGTCTG | |
| Mas1 | Forward | ACCTCCAGCAGAAATGCCTC |
| Reverse | TCCCATTCTCCACAAAGCCC | |
| Il‐6 | Forward | GAGACTTCCATCCAGTTGCCT |
| Reverse | TCCTCTGTGAAGTCTCCTCTCC | |
| Nfκb1 | Forward | ACTGTCTGCCTCTCTCGTCT |
| Reverse | CCGTGGGGCATTTTGTTCAG | |
| Tnfα | Forward | CGGGCAGGTCTACTTTGGAG |
| Reverse | ACCCTGAGCCATAATCCCCT | |
| Nlrp3 | Forward | CAAGGCTGCTATCTGGAGGAA |
| Reverse | TTCTCGGGCGGGTAATCTTC | |
| Stat3 | Forward | TTGTCGGTTGGAGGTGTGAG |
| Reverse | AAGTGGGAAAGGAAGGCAGG |