FIG. 5.

Influence of preincubation of sporozoites with MAb 11D5/H3 on their subsequent intracellular localization. Sporozoites were treated with 50 μg of MAb 11D5/H3 per ml before inoculation onto cell cultures. An isotype-matched irrelevant MAb and the sporozoite-reactive MAb 2C6/E9 were used, both at 50 μg/ml, and medium alone served as controls. The number of intracytosolic stages was determined as described before and expressed as a percent of all intracellular parasites. The graph summarizes three independent experiments whereby three coverslips were examined for each treatment and 200 to 300 sporozoites were evaluated on each coverslip. Data are shown as dot plots, and bars indicate the median. Kruskal-Wallis ANOVA and pairwise multiple comparison procedures (Student-Newman-Keuls method) revealed that the median for 11D5/H3 treatment was significantly higher than those of all other treatments (d.f. = 3; H = 19.096; P < 0.001); no statistically significant differences occurred between the other groups.