Figure 2.

Knockout of OsGLPs results in the growth inhibition of rice seedlings. (A) Construction and identification of mutants. Two gRNAs were designed based on the exons of OsGLP8-2 and OsGLP8-11 near the ATG. Light blue and dark blue indicate successful sequence matching. Wavy lines are sequencing peaks. (B) Identification of OsGLP8-2-overexpressing lines. (C) Phenotypes of two-week-old WT (wild type) and transgenic seedlings grown for 5 days under normal conditions, 10 μmol L⁻¹ CuSO₄ treatment, or 25 μmol L⁻¹ CdCl₂ treatment. Scale bar = 3 cm. (D, E) Root elongation of WT and transgenic plants. (F, G) Dry weight of WT and transgenic plants. Values are the mean ± SD; n = 3. Different letters indicate a difference of p ≤ 0.05 by the LSD test.