Figure 4.

Analysis of D_H use, J_H use, and germline status of young and aged PtC+CD5+ B-1 cells. PtC+CD5+ B-1 cells were single-cell sorted from the peritoneal cavity or spleen of young and aged female BALB/c-ByJ mice (as presented in Figure 2 ). (A) The percent of D_H gene segment usage. (B) The percent of J_H gene segment usage. (C) The percent of sequences containing zero N-additions (light grey bars) or 1 or more N-additions (dark grey hashed bars) at both junctions is shown with replicate sequences included in the analysis. For direct comparison of females and males, previously published (24) N- addition analysis of young and aged male PtC+ CD5+ B-1 cells is included. Results are based on 4 independent experiments with sequences combined fromeach independent experiment (n=11 for 3-month-old mice, n=15 for 23-26-month-old female mice). Statistics used: 2x2 and 2x4 chi-square test. Asterisks for p values: **p<0.01, ****p<0.0001.