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. 2023 Jan 12;13:1100092. doi: 10.3389/fimmu.2022.1100092

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Copyright © 2023 Pereiro, Rey-Campos, Figueras and Novoa

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of long-term exposure to SMX+CLA on the number of innate immune cells. (A) Representative images of double transgenic Tg(mpeg:mCherry/mpx:GFP) zebrafish larvae exposed to vehicle alone (DMSO) or SMX+CLA for 7 days. Scale bar: 500 µm. (B) Macrophage and neutrophil counts in DMSO- and SMX+CLA-treated larvae. Statistically significant differences are displayed as **** (p<0.0001) and ** (p<0.01). (C) Expression level of the macrophage (marco, mpeg1.1) and neutrophil (mpx) gene cell markers in DMSO- and SMX+CLA-treated larvae for 7 days and (D) in the intestine of adult zebrafish exposed to the treatments for 2 weeks. Statistically significant differences are displayed as * (p<0.05).