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. 2023 Jan 12;13:1085918. doi: 10.3389/fmicb.2022.1085918

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Copyright © 2023 Habibi Arejan, Ensinck, Diacovich, Patel, Quintanilla, Emami Saleh, Gramajo and Boutte.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Wag31 does not regulate FtsI in logarithmic phase through residues NSD46-48. (A) Representative plates from the Mycobacterial Protein Fragment Complementation two-hybrid assay. Indicated strains were struck out on plates with and without trimethoprim (+TMP/ -TMP). (B) Doubling times of Msmeg cells expressing WT or wag31 NSD46-8AAA mutant. (C) Cell lengths and (D) Cell width of the wag31 NSD46-8AAA strain. Black bars are at the mean. (E) Relative septal and polar HADA (F) intensity of Wag31 WT and Wag31 NSD46-8AAA. ns, p > 0.05, *p = <0.05, ***p = <0.0005, ****p = <0.0001. p-values were calculated by unpaired t-test.