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The effect of S2303 on Staphylococcus aureus FtsZ assembly in vitro. Polymerization of SaFtsZ (11 μM) was initiated by 1 mM GTP and the assembly of FtsZ in the absence [1% Dimethyl sulfoxide (DMSO), dark] or presence of 4 μg/mL (red) and 8 μg/mL (blue) S2303 was monitored by 90° angle light scattering. Appropriate blanks were subtracted from all the traces; the experiment was repeated in triplicate.
