Table 1.

Primer sequences of the porcine reference genes (RPL4, PPIA, B2M, HPRT1, and TBP) and target genes (CD28, CTLA4, PD1, PDL1, CD226, TIGIT, TIM3, LAG3, IDO1, TNFRSF9, SELL, ICOS, and CD40).

Genes	Type	Sequences	Reference
RPL4	Reference gene	F 5′-CAAGAGTAACTACAACCTTC-3′	Nygard et al. (2007)
		R 5′-GAACTCTACGATGAATCTTC-3′
PPIA	Reference gene	F 5′-CGCGTCTCCTTCGAGCTGTTT-3′	Self-designed
		R 5′-GCGTGTGAAGTCACCACCCT-3´
B2M	Reference gene	F 5′-ACTTTTCACACCGCTCCAGT-3´	Self-designed
		R 5′-CGGATGGAACCCAGATACAT-3´
HPRT1	Reference gene	F 5′-GGACTTGAATCATGTTTGTG-3´	Nygard et al. (2007)
		R 5′-CAGATGTTTCCAAACTCAAC-3′
TBP	Reference gene	F 5′-ACGTTCGGTTTAGGTTGCAG −3′	Uddin et al. (2011)
		R 5′-GCAGCACAGTACGAGCAACT-3′
CD28	Target gene	F 5′-CCCCTCAATTCAAGTAACAGGAAAC-3′	Self-designed
		R 5′-ATGCCCGGAACTCCTTTGAG-3′
CTLA4	Target gene	F 5′-TCTTCATCCCTGTCTTCTCCAAA-3′	Yue et al. (2014)
		R 5′-GCAGACCCATACTCACACACAAA-3′
PD1	Target gene	F 5′-AGCCCAAGCACTTCATCCTC-3′	Self-designed
		R 5′-TGTGGAAGTCTCGTCCGTTG-3′
PDL1	Target gene	F 5′-GTGGAAAAATGTGGCAGCCG-3′	Self-designed
		R 5′-TGCTTAGCCCTGACGAACTC-3′
CD226	Target gene	F 5′-TGGAGGAGCAGCTTTGTTGTT-3′	Self-designed
		R 5′-TTTCTGTCTCCTTCTCCTTCTCTT-3′
TIGIT	Target gene	F 5′-TCACGTGGGCCAGAAAGAAATC-3′	Self-designed
		R 5′-CCAATGCTGGCGGGTATTCT-3′
TIM3	Target gene	F 5′-TTCGACGGGAGCAGTAAAGC-3′	Self-designed
		R 5′-AGGGCAGGACACAGTCAAAG-3′
LAG3	Target gene	F 5′-CTCCTCCTGCTCCTTTTGGTT-3′	Yue et al. (2014)
		R 5′-CAGCTCCCCAGTCTTGCTCT-3′
IDO1	Target gene	F 5′-GGCACTTGATTGGTGGTCTC-3′	Bacou et al. (2017)
		R 5′-GCAATCCAAGCATCGTAAGG-3′
SELL	Target gene	F 5′-CCTAGTCCGATATGTCAAAAACTGG-3′	Self-designed
		R 5′-TCATCCATGCTTCTCTGAGACTT-3′
TNFRSF9	Target gene	F 5′-TTGCCAGCAAGGTCAAGAGT-3′	Self-designed
		R 5′-AGCCAAAGAACAGTCCGTCC-3′
ICOS	Target gene	F 5′-CCAGCGTGCATGACCCTAAT-3′	Self-designed
		R 5′-TTGCGGGTCACATCTGTTGG-3′
CD40	Target gene	F 5′-GTTTGAAGACCTGGTCAAGGGTA-3′	Self-designed
		R 5′-CATGTGCCGCAATTTGAGGAT-3′