FIG. 4.

Digital analysis of fluorescence microscopy showing induction of GFP reported as a ratio of GFP (green fluorescence) to DNA (blue fluorescence). Promoter constructs were cultured in media alone and with HMEC-1 as indicated for 24 h before images were captured by digital fluorescence imaging. B. henselae clones were incubated in cell culture media alone (▧) or cocultured with HMEC-1 (▪). B. henselae GFP reporter constructs analyzed were B. henselae 882Str/pANT3 (A), B. henselae pVBGFPR/882Str (B), B. henselae pVBGFPF/882Str (C), and B. henselae pVBDEL/882Str (D).