TABLE 1.
Description of plasmids and bacterial strains
| Plasmid or strain | Application or genotype | Reference |
|---|---|---|
| Plasmids | ||
| pUC19 | Ampr:lacZα | 42 |
| pICB.4D7 | pUC19 with 4.0-kb EcoRI B. henselae Houston-1 genomic DNA fragment isolated by virB2 hybridization | This work |
| pCB182 | Promoterless lacZ galK:Ampr | 30 |
| pCBlacZ | pCB182 with B. henselae htr P1 promoter | |
| pSIR4 | pCB182 with E. coli lacZ promoter in forward orientation | |
| pANT3 | Promoterless gfpmut3:Kanr | 19 |
| pVirBF | pCB182 with B. henselae virB promoter region in the forward orientation | This work |
| pVirBR | pCB182 with B. henselae virB promoter region in the reverse orientation | This work |
| pVBGFPF | pANT3 with B. henselae virB promoter region in the forward orientation | This work |
| pVBGFPR | pANT3 with B. henselae virB promoter region in the reverse orientation | This work |
| pVBDEL | pANT3 with 87-bp 5′ deletion of the B. henselae virB promoter region | This work |
| Strains | ||
| B. henselae | ||
| Houston-1 | ATCC 49882 | 24 |
| 882Str | Streptomycin-resistant laboratory-adapted strain of ATCC 49882 | 19 |
| E. coli | ||
| DH5α | endA1 hsdR17 (rK−mK+) glnV44 thi-1 recA1 gyrA (Nalr) relA1 Δ(lacIZYA-argF)U169 deoR (φ80dlacΔ[lacZ]M15) | |
| JM109 | F′ traD36 lacIq Δ(lacZ)M15 proA+B+/e14−(McrA−) Δ(lac-proAB) thi gyrA96 (Nalr) endA1 hsdR17 (rK−mK+) relA1 supE44 recA1 | |
| CB454 | F− ΔlacZ−lacY+galK rpsL thi recA56 | 30 |