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. 2023 Jan 6;95(3):1933–1939. doi: 10.1021/acs.analchem.2c04106

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© 2023 The Authors. Published by American Chemical Society

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Workflow for detecting and identifying EV populations form single cells. (A) Using fluorescently conjugated mAbs and four-color TIRFM, we image immobilized EVs (B) in the direct neighborhood of the cells, which are prevented from cross-contamination with EVs from other cells. (C) Multicolor TIRFM images of secreted and immobilized EVs. (D) Customized Matlab imaging processing allows for quantifying and identifying EVs per image. Note that the histograms display raw data (detected signals per image).