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. 2023 Jan 10;95(3):1958–1966. doi: 10.1021/acs.analchem.2c04222

Figure 3.

Figure 3

Rolling behavior of KG1a cells on CHO-E monolayer. (a) Time-lapse bright-field images of single KG1a cells rolling over a CHO-E monolayer at a shear stress of 2 dyne cm–2. (b) Frame-to-frame single-cell velocity over 70 s time period. (c) Number of adhered then rolled KG1a cells over CHO-E and CHO-K monolayers in the presence or absence of EDTA. (d) Effect of the blocking of active binding sites on E-selectin on the time-averaged rolling velocity of KG1a cells on the CHO-E monolayer. The active binding sites were blocked by two different blocking antibodies, BB2G and H18-7. (e) Time-averaged rolling velocity of KG1a cells over CHO-E monolayer and rh E-selectin deposited on a glass surface with different wall shear stress values. Error bars represent ±SD determined by n = 3 independent experiments in panels (c)–(e).